mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00479543" target="_blank" >RIV/68378041:_____/17:00479543 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.ejcb.2017.05.001" target="_blank" >http://dx.doi.org/10.1016/j.ejcb.2017.05.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejcb.2017.05.001" target="_blank" >10.1016/j.ejcb.2017.05.001</a>

Result language

angličtina

Original language name

mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane

Original language description

We describe a novel mechanism of mRNA decay regulation, which takes place under the conditions of glucose deprivation in the yeast Saccharomyces cerevisiae. The regulation is based on temporally stable sequestration of the main 5'-3' mRNA exoribonuclease Xrn1 at the eisosome, a plasma membrane-associated protein complex organizing a specialized membrane microdomain. As documented by monitoring the decay of a specific mRNA substrate in time, Xrn1-mediated mRNA degradation ceases during the accumulation of Xrn1 at eisosome, but the eisosome-associated Xrn1 retains its functionality and can be re-activated when released to cytoplasm following the addition of glucose. In cells lacking the eisosome organizer Pil1, Xrn1 does not associate with the plasma membrane and its activity is preserved till the stationary phase. Thus, properly assembled eisosome is necessary for this kind of Xrn1 regulation, which occurs in a liquid culture as well as in a differentiated colony.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/GA15-10641S" target="_blank" >GA15-10641S: Specific plasma membrane microdomains in regulation of aging</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Cell Biology

ISSN

0171-9335

e-ISSN

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Volume of the periodical

96

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

591-599

UT code for WoS article

000414276700010

EID of the result in the Scopus database

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