Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00486485" target="_blank" >RIV/68378041:_____/17:00486485 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.cryobiol.2017.04.001" target="_blank" >http://dx.doi.org/10.1016/j.cryobiol.2017.04.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cryobiol.2017.04.001" target="_blank" >10.1016/j.cryobiol.2017.04.001</a>
Alternative languages
Result language
angličtina
Original language name
Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts
Original language description
The generation of an off-the-shelf in vitro engineered living tissue graft will likely require cryopreservation. However, the efficient addition and removal of cryoprotective agents (CPA) to cells throughout the volume of a three-dimensional (3D) tissue graft remains a significant challenge. In this work, we assessed whether a perfusion bioreactor-based method could be used to improve the viability of cryopreserved mesenchymal stromal cell- (MSC) based tissue constructs as compared to using conventional diffusion-based methods. The bioreactor was first used to saturate 3D constructs with CPA under perfused flow. Following cryopreservation, the bioreactor was then also used for the efficient removal of the CPA from the 3D tissues. We demonstrate that addition and removal of CPA under perfused flow significantly increased the viability of MSC within cryopreserved 3D tissue constructs as compared to conventional diffusion-based methods. (C) 2017 Elsevier Inc. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cryobiology
ISSN
0011-2240
e-ISSN
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Volume of the periodical
76
Issue of the periodical within the volume
jun.
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
150-153
UT code for WoS article
000403193400020
EID of the result in the Scopus database
2-s2.0-85017228290