Kinetics of ROS generation induced by polycyclic aromatic hydrocarbons and organic extracts from ambient air particulate matter in model human lung cell lines

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F18%3A00493296" target="_blank" >RIV/68378041:_____/18:00493296 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.mrgentox.2018.01.006" target="_blank" >http://dx.doi.org/10.1016/j.mrgentox.2018.01.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mrgentox.2018.01.006" target="_blank" >10.1016/j.mrgentox.2018.01.006</a>

Result language

angličtina

Original language name

Kinetics of ROS generation induced by polycyclic aromatic hydrocarbons and organic extracts from ambient air particulate matter in model human lung cell lines

Original language description

Polycyclic aromatic hydrocarbons /PAHs/ associated with particulate matter /PM/ may induce oxidative damage via reactive oxygen species /ROS/ generation. However, the kinetics of ROS production and the link with antioxidant response induction has not been well studied. To elucidate the differences in oxidative potential of individual PAH compounds and extractable organic matter /EOM/ from PM containing various PAH mixtures, we studied ROS formation and antioxidant response /total antioxidant capacity /TAC/ and expression of HMOXI and TXNRD1/ in human alveolar basal epithelial cells /A549 cells/ and human embryonic lung fibroblasts /HEL12469 cells/. We treated the cells with three concentrations of model PAHs /benzo/a/pyrene, B/a/P, 3-nitrobenzanthrone, 3-NBA/ and EOM from PM < 2.5 mu m /PM2.5/. ROS levels were evaluated at 8 time intervals /30 min-24 h/. In both cell lines, B/a/P treatment was associated with a time-dependent decrease of ROS levels. This trend was more pronounced in HEL12469 cells and was accompanied by increased TAC. A similar response was observed upon 3-NBA treatment in HEL12469 cells. In A549 cells, however, this compound significantly increased superoxide levels. This response was accompanied by the decrease of TAC as well as HMOXI and TXNRD1 expression. In both cell lines, a short-time exposure to EOMs tended to increase ROS levels, while a marked decrease was observed after longer treatment periods. This was accompanied by the induction of HMOX1 and TXNRD1 expression in HEL12469 cells and increased TAC in A549 cells. In summary, our data indicate that in the studied cell lines B/a/P and EOMs caused a time-dependent decrease of intracellular ROS levels, probably due to the activation of the antioxidant response. This response was not detected in A549 cells following 3-NBA treatment, which acted as a strong superoxide inducer. Pro-oxidant properties of EOMs are limited to short-time exposure periods.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA16-14631S" target="_blank" >GA16-14631S: The study of processes associated with lipid peroxidation in model human cell lines</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

ISSN

1383-5718

e-ISSN

—

Volume of the periodical

827

Issue of the periodical within the volume

mar

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

50-58

UT code for WoS article

000428494500006

EID of the result in the Scopus database

2-s2.0-85041483440