The Transdifferentiation of Adipose Tissue-Derived Mesenchymal Stem Cells Info Neural-like Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F18%3A00500075" target="_blank" >RIV/68378041:_____/18:00500075 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The Transdifferentiation of Adipose Tissue-Derived Mesenchymal Stem Cells Info Neural-like Cells
Original language description
The process of transdifferentiation of mesenchymal stem cells into neuron-like phenotype is a challenging and promising approach in the treatment of neurological diseases. To achieve neuron-like differentiation, we used human adipose tissue-derived mesenchymal stem cells (MSCs), which underwent long-term cultivation in the presence of growth factors, brain tissue postnuclear supernatant, supernatant from phytohemaglutitin (PHA)-stimulated lymphocytes and electrostimulation under physiological conditions. The differentiated cells showed an increased relative gene expression of neuron-specific markers (light neurofilament (Nf-L), neural cadherin (NCAM), beta-III tubulin (Tubb3) and glutamic acid decarboxylase (GAD)) and the presence of medium neurofilament (Nf-M) and Tubb-3 proteins. However, subsequent electrophysiological measurement did not reveal sensitivity to neurotransmitters and no spontaneous postsynaptic currents were observed. In conclusion, despite the expression of some neural specific markers, the current protocol was unsuccessful in achieving the complete transdifferentiation of MSCs to neuron-like cells.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/LO1309" target="_blank" >LO1309: Cell Therapy and Tissue Repair</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Nanostructures in Biomedicine: Their Regenerative and toxic properties
ISBN
978-80-906655-7-6
Number of pages of the result
7
Pages from-to
92-98
Number of pages of the book
198
Publisher name
Proxima Tisk, s.r.o.
Place of publication
Praha 3
UT code for WoS chapter
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