Needleless electrospun and centrifugal spun poly-epsilon-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518445" target="_blank" >RIV/68378041:_____/19:00518445 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21220/19:00327459 RIV/68407700:21720/19:00327459 RIV/00216208:11310/19:10394293 RIV/00216208:11130/19:10394293
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0928493118302625?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0928493118302625?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.msec.2018.12.069" target="_blank" >10.1016/j.msec.2018.12.069</a>
Alternative languages
Result language
angličtina
Original language name
Needleless electrospun and centrifugal spun poly-epsilon-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs
Original language description
The biofunctionalization of scaffolds for tissue engineering is crucial to improve the results of regenerative therapies. This study compared the effect of platelet-functionalization of 2D electrospun and 3D centrifugal spun scaffolds on the osteogenic potential of hMSCs. Scaffolds prepared from poly-epsilon-caprolactone, using electrospinning and centrifugal spinning technology, were functionalized using five different concentrations of platelets. Cell proliferation, metabolic activity and osteogenic differentiation were tested using hMSCs cultured in differential and non-differential medium. The porous 3D structure of the centrifugal spun fibers resulted in higher cell proliferation. Furthermore, the functionalization of the scaffolds with platelets resulted in a dose dependent increase in cell metabolic activity, proliferation and production of an osteogenic marker alkaline phosphatase. The effect was further promoted by culture in an osteogenic differential medium. The increase in combination of both platelets and osteogenic media shows an improved osteoinduction by platelets in environments rich in inorganic phosphate and ascorbate. Nevertheless, the results of the study showed that the optimal concentration of platelets for induction of hMSC osteogenesis is in the range of 900-3000 x 10(9) platelets/L. The study determines the potential of electrospun and centrifugal spun fibers with adhered platelets, for use in bone tissue engineering.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials Science & Engineering C-Materials for Biological Applications
ISSN
0928-4931
e-ISSN
—
Volume of the periodical
97
Issue of the periodical within the volume
apr.
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
567-575
UT code for WoS article
000457952800054
EID of the result in the Scopus database
2-s2.0-85059050644