New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F20%3A00536980" target="_blank" >RIV/68378041:_____/20:00536980 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/20:10417374 RIV/00216208:11150/20:10417374 RIV/00179906:_____/20:10417374

Result on the web

<a href="https://www.mdpi.com/2227-9059/8/11/477" target="_blank" >https://www.mdpi.com/2227-9059/8/11/477</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biomedicines8110477" target="_blank" >10.3390/biomedicines8110477</a>

Result language

angličtina

Original language name

New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats

Original language description

Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty's catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 mu L of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 mu L resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 mu L resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 mu L.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMEDICINES

ISSN

2227-9059

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

477

UT code for WoS article

000592948400001

EID of the result in the Scopus database

2-s2.0-85096070030