Long-Term Cultures of Spinal Cord Interneurons
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F22%3A00566895" target="_blank" >RIV/68378041:_____/22:00566895 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/22:10441303
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fncel.2022.827628/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fncel.2022.827628/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fncel.2022.827628" target="_blank" >10.3389/fncel.2022.827628</a>
Alternative languages
Result language
angličtina
Original language name
Long-Term Cultures of Spinal Cord Interneurons
Original language description
Spinal cord interneurons (SpINs) are highly diverse population of neurons that play a significant role in circuit reorganization and spontaneous recovery after spinal cord injury. Regeneration of SpIN axons across rodent spinal injuries has been demonstrated after modification of the environment and neurotrophin treatment, but development of methods to enhance the intrinsic regenerative ability of SpINs is needed. There is a lack of described in vitro models of spinal cord neurons in which to develop new regeneration treatments. For this reason, we developed a new model of mouse primary spinal cord neuronal culture in which to analyze maturation, morphology, physiology, connectivity and regeneration of identified interneurons. Isolated from E14 mice, the neurons mature over 15 days in vitro, demonstrated by expression of maturity markers, electrophysiological patch-clamp recordings, and formation of synapses. The neurons express markers of SpINs, including Tlx3, Lmx1b, Lbx1, Chx10, and Pax2. The neurons demonstrate distinct morphologies and some form perineuronal nets in long-term cultivation. Live neurons in various maturation stages were axotomized, using a 900 nm multiphoton laser and their fate was observed overnight. The percentage of axons that regenerated declined with neuronal maturity. This model of SpINs will be a valuable tool in future regenerative, developmental, and functional studies alongside existing models using cortical or hippocampal neurons.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cellular Neuroscience
ISSN
1662-5102
e-ISSN
1662-5102
Volume of the periodical
16
Issue of the periodical within the volume
feb.
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
827628
UT code for WoS article
000760448300001
EID of the result in the Scopus database
2-s2.0-85125043940