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Long-Term Cultures of Spinal Cord Interneurons

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F22%3A00566895" target="_blank" >RIV/68378041:_____/22:00566895 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11130/22:10441303

  • Result on the web

    <a href="https://www.frontiersin.org/articles/10.3389/fncel.2022.827628/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fncel.2022.827628/full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fncel.2022.827628" target="_blank" >10.3389/fncel.2022.827628</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Long-Term Cultures of Spinal Cord Interneurons

  • Original language description

    Spinal cord interneurons (SpINs) are highly diverse population of neurons that play a significant role in circuit reorganization and spontaneous recovery after spinal cord injury. Regeneration of SpIN axons across rodent spinal injuries has been demonstrated after modification of the environment and neurotrophin treatment, but development of methods to enhance the intrinsic regenerative ability of SpINs is needed. There is a lack of described in vitro models of spinal cord neurons in which to develop new regeneration treatments. For this reason, we developed a new model of mouse primary spinal cord neuronal culture in which to analyze maturation, morphology, physiology, connectivity and regeneration of identified interneurons. Isolated from E14 mice, the neurons mature over 15 days in vitro, demonstrated by expression of maturity markers, electrophysiological patch-clamp recordings, and formation of synapses. The neurons express markers of SpINs, including Tlx3, Lmx1b, Lbx1, Chx10, and Pax2. The neurons demonstrate distinct morphologies and some form perineuronal nets in long-term cultivation. Live neurons in various maturation stages were axotomized, using a 900 nm multiphoton laser and their fate was observed overnight. The percentage of axons that regenerated declined with neuronal maturity. This model of SpINs will be a valuable tool in future regenerative, developmental, and functional studies alongside existing models using cortical or hippocampal neurons.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30103 - Neurosciences (including psychophysiology)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Cellular Neuroscience

  • ISSN

    1662-5102

  • e-ISSN

    1662-5102

  • Volume of the periodical

    16

  • Issue of the periodical within the volume

    feb.

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    17

  • Pages from-to

    827628

  • UT code for WoS article

    000760448300001

  • EID of the result in the Scopus database

    2-s2.0-85125043940