Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F23%3A00571245" target="_blank" >RIV/68378041:_____/23:00571245 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/23:00571245 RIV/00216208:11130/23:10459037 RIV/00216208:11310/23:10459037 RIV/60461373:22310/23:43928170
Result on the web
<a href="https://www.nature.com/articles/s41598-023-33608-y" target="_blank" >https://www.nature.com/articles/s41598-023-33608-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-023-33608-y" target="_blank" >10.1038/s41598-023-33608-y</a>
Alternative languages
Result language
angličtina
Original language name
Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology
Original language description
The role of glia in amyotrophic lateral sclerosis (ALS) is undeniable. Their disease-related activity has been extensively studied in the spinal cord, but only partly in the brain. We present herein a comprehensive study of glia in the cortex of SOD1(G93A) mice—a widely used model of ALS. Using single-cell RNA sequencing (scRNA-seq) and immunohistochemistry, we inspected astrocytes, microglia, and oligodendrocytes, in four stages of the disease, respecting the factor of sex. We report minimal changes of glia throughout the disease progression and regardless of sex. Pseudobulk and single-cell analyses revealed subtle disease-related transcriptional alterations at the end-stage in microglia and oligodendrocytes, which were supported by immunohistochemistry. Therefore, our data support the hypothesis that the SOD1(G93A) mouse cortex does not recapitulate the disease in patients, and we recommend the use of a different model for future studies of the cortical ALS pathology.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
13
Issue of the periodical within the volume
april
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
6538
UT code for WoS article
000989734900016
EID of the result in the Scopus database
2-s2.0-85153548229