Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F24%3A00587538" target="_blank" >RIV/68378041:_____/24:00587538 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/24:10484997
Result on the web
<a href="https://www.nature.com/articles/s41565-024-01711-4" target="_blank" >https://www.nature.com/articles/s41565-024-01711-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41565-024-01711-4" target="_blank" >10.1038/s41565-024-01711-4</a>
Alternative languages
Result language
angličtina
Original language name
Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes
Original language description
Astrocytes are responsible for maintaining homoeostasis and cognitive functions through calcium signalling, a process that is altered in brain diseases. Current bioelectronic tools are designed to study neurons and are not suitable for controlling calcium signals in astrocytes. Here, we show that electrical stimulation of astrocytes using electrodes coated with graphene oxide and reduced graphene oxide induces respectively a slow response to calcium, mediated by external calcium influx, and a sharp one, exclusively due to calcium release from intracellular stores. Our results suggest that the different conductivities of the substrate influence the electric field at the cell–electrolyte or cell–material interfaces, favouring different signalling events in vitro and ex vivo. Patch-clamp, voltage-sensitive dye and calcium imaging data support the proposed model. In summary, we provide evidence of a simple tool to selectively control distinct calcium signals in brain astrocytes for straightforward investigations in neuroscience and bioelectronic medicine.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Nanotechnology
ISSN
1748-3387
e-ISSN
1748-3395
Volume of the periodical
19
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
1344-1353
UT code for WoS article
001268978700002
EID of the result in the Scopus database
2-s2.0-85198123860