The timing of pronuclear transfer critically affects the developmental competence and quality of embryos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F24%3A00588269" target="_blank" >RIV/68378041:_____/24:00588269 - isvavai.cz</a>

Alternative codes found

RIV/00027014:_____/24:10006015

Result on the web

<a href="https://academic.oup.com/molehr/article-abstract/30/7/gaae024/7712434?redirectedFrom=fulltext&login=false" target="_blank" >https://academic.oup.com/molehr/article-abstract/30/7/gaae024/7712434?redirectedFrom=fulltext&login=false</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/molehr/gaae024" target="_blank" >10.1093/molehr/gaae024</a>

Result language

angličtina

Original language name

The timing of pronuclear transfer critically affects the developmental competence and quality of embryos

Original language description

Pronuclear transfer has been successfully used in human-assisted reproduction to suppress the adverse effects of a defective oocyte cytoplasm or to bypass an idiopathic developmental arrest. However, the effects of the initial parental genome remodelling in a defective cytoplasm on the subsequent development after pronucleus transfer have not been systematically studied. By performing pronuclear transfer in pre-replication and post-replication mouse embryos, we show that the timing of the procedure plays a critical role. Although apparently morphologically normal blastocysts were obtained in both pre- and post-replication pronuclear transfer groups, post-replication pronuclear transfer led to a decrease in developmental competence and profound changes in embryonic gene expression. By inhibiting the replication in the abnormal cytoplasm before pronuclear transfer into a healthy cytoplasm, the developmental potential of embryos could be largely restored. This shows that the conditions under which the first embryonic replication occurs strongly influence developmental potential. Although pronuclear transfer is the method of choice for mitigating the impact of a faulty oocyte cytoplasm on early development, our results show that the timing of this intervention should be restricted to the pre-replication phase.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

<a href="/en/project/GA20-04465S" target="_blank" >GA20-04465S: Understanding the factors critical for successful paternal genome remodelling: Dissecting the role of oocyte components</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Human Reproduction

ISSN

1360-9947

e-ISSN

1460-2407

Volume of the periodical

30

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

gaae024

UT code for WoS article

001273657900001

EID of the result in the Scopus database

2-s2.0-85199776139