Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00617182" target="_blank" >RIV/68378041:_____/25:00617182 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/26:0193562
Result on the web
<a href="https://accscience.com/journal/IJB/articles/online_first/4158" target="_blank" >https://accscience.com/journal/IJB/articles/online_first/4158</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.36922/ijb.5175" target="_blank" >10.36922/ijb.5175</a>
Alternative languages
Result language
angličtina
Original language name
Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells
Original language description
Bone tissue engineering requires scaffolds with three-dimensional (3D) structures that facilitate vascularization and new tissue growth. 3D printing, especially through fused deposition modeling (FDM), has emerged as an effective method for creating complex structures with high reproducibility. Early research in this area demonstrated the potential of poly(ε-caprolactone) (PCL) and poly(L-lactide) (PLLA) scaffolds for bone regeneration. Recently, polylactide (PLA) and polyhydroxyalkanoates (PHAs) have garnered attention for their biocompatibility and ability to support cell proliferation. Among PHAs, poly(3-hydroxybutyrate) (PHB) shows promise due to its intrinsic biocompatibility and resorbability, making it a candidate for FDM-based scaffold fabrication. In the presented study, we aim to develop and optimize a biocompatible PHB-based composite material for bone tissue engineering, incorporating PLA, hydroxyapatite, and the plasticizer Syncroflex 3114 to enhance mechanical properties and printability. This composite was processed into filaments for 3D printing and characterized through thermal, mechanical, and biological evaluations. Using a design of experiment approach, we investigated factors such as temperature performance, warping, degradation, and strength to determine the optimal composition for use in tissue engineering. Four optimal mixture compositions fulfilling the optimization criteria of having the most suitable properties for bone tissue engineering, namely the best printability and maximum mechanical properties, were obtained. The mixtures were optimized specifically for minimum warping coefficient (0.5), maximum flexural strength (66.9 MPa), maximum compression modulus (2.4 GPa), and maximum compression modulus (2.3 GPa) with a warping coefficient of no more than 1 at the same time. In conclusion, the study shows a new possible way to effectively develop and test 3D-printed PHB-based scaffolds with specifically optimized material properties.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
<a href="/en/project/EH22_008%2F0004562" target="_blank" >EH22_008/0004562: Excellent Research in Regenerative Medicine</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Bioprinting
ISSN
2424-7723
e-ISSN
2424-8002
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
SG - SINGAPORE
Number of pages
18
Pages from-to
400-417
UT code for WoS article
001501058400002
EID of the result in the Scopus database
2-s2.0-85218863490