SMAD4 promotes somatic-germline contact during murine oocyte growth

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A90250%2F24%3A00637182" target="_blank" >RIV/68378050:90250/24:00637182 - isvavai.cz</a>

Result on the web

<a href="https://elifesciences.org/articles/91798" target="_blank" >https://elifesciences.org/articles/91798</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7554/eLife.91798" target="_blank" >10.7554/eLife.91798</a>

Result language

angličtina

Original language name

SMAD4 promotes somatic-germline contact during murine oocyte growth

Original language description

Development of the mammalian oocyte requires physical contact with the surrounding granulosa cells of the follicle, which provide it with essential nutrients and regulatory signals. This contact is achieved through specialized filopodia, termed transzonal projections (TZPs), that extend from the granulosa cells to the oocyte surface. Transforming growth factor (TGF beta) family ligands produced by the oocyte increase the number of TZPs, but how they do so is unknown. Using an inducible Cre recombinase strategy together with expression of green fluorescent protein to verify Cre activity in individual cells, we examined the effect of depleting the canonical TGF beta mediator, SMAD4, in mouse granulosa cells. We observed a 20-50% decrease in the total number of TZPs in SMAD4-depleted granulosa cell-oocyte complexes, and a 50% decrease in the number of newly generated TZPs when the granulosa cells were reaggregated with wild-type oocytes. Three-dimensional image analysis revealed that TZPs of SMAD4-depleted cells were longer than controls and more frequently oriented towards the oocyte. Strikingly, the transmembrane proteins, N-cadherin and Notch2, were reduced by 50% in SMAD4-depleted cells. SMAD4 may thus modulate a network of cell adhesion proteins that stabilize the attachment of TZPs to the oocyte, thereby amplifying signalling between the two cell types.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

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Continuities

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Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

eLife

ISSN

2050-084X

e-ISSN

2050-084X

Volume of the periodical

13

Issue of the periodical within the volume

May

Country of publishing house

GB - UNITED KINGDOM

Number of pages

21

Pages from-to

RP91798

UT code for WoS article

001236747400001

EID of the result in the Scopus database

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