Analysis of mouse polyomavirus mutants with lesions in the minor capsid proteins.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F02%3A23033132" target="_blank" >RIV/68378050:_____/02:23033132 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/02:11825

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Analysis of mouse polyomavirus mutants with lesions in the minor capsid proteins.

Original language description

Polyomavirus mutants E, Q and H, expressing non-myristylated VP2, were generated by replacing the N-terminal glycine residue with glutamic acid, glutamine or histidine, respectively. Viruses mutated in either VP2 or VP3 translation codons were also prepared. All mutated genomes, when transfected into murine host cells, gave rise to viral particles. Infectivity of VP2- and VP3- viruses was dramatically diminished, indicative of defects in the early stages of infection. No differences in number of cells expressing early or late viral antigens were observed between wild-type and E or Q myr- viruses. Furthermore, no delay in virus DNA replication was detected.The infectivity of mutants was affected not only by loss of the myristyl group on VP2, but also, and to a greater extent, by alteration of the N-terminal amino acid composition.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F00%2F0271" target="_blank" >GA204/00/0271: Ultrastructural studies of polyomavirus antigen interactions with substructures of virus infected cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of General Virology

ISSN

0022-1317

e-ISSN

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Volume of the periodical

83

Issue of the periodical within the volume

N/A

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

2309-2319

UT code for WoS article

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EID of the result in the Scopus database

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