Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F03%3A23033091" target="_blank" >RIV/68378050:_____/03:23033091 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis.

Original language description

Video Rate Confocal Laser Scanning Microscope (VRCLSM) Odyssey (Noran, Middleton, WI, USA) at the highest spatial and temporal resolution of back scattered light (BSL) imaging enabled regular observation of fast intracellular motion (FIM) first revealedin living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motionand a known type of motion such as cell marginal ruffling compared with FIM. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysisof FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color coded images only.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

—

Project

<a href="/en/project/GA304%2F99%2F0368" target="_blank" >GA304/99/0368: The significance of fast intracellular motile reactions in living cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SCANNING

ISSN

0161-0457

e-ISSN

—

Volume of the periodical

25

Issue of the periodical within the volume

N/A

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

230-239

UT code for WoS article

—

EID of the result in the Scopus database

—