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Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F04%3A00109714" target="_blank" >RIV/68378050:_____/04:00109714 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding

  • Original language description

    The integrity of sperm acrosome is required for normal sperm-egg interaction. Antibodies to acrosomal proteins are still frequently utilized to check the acrosomal state. We studied acrosomal proteins in boar and human spermatozoa by using of Hs-8 and Hs-14 monoclonal antibodies (MoAbs) which were prepared in our laboratory.The intra-acrosomal proteins recognized by Hs-8 and Hs-14 MoAbs are stable upon capacitation and even after induction of acrosome reaction. The role of pHs-8 and pHs-14 proteins in sperm-egg binding was investigated by in vitro binding assay of boar spermatozoa and porcine oocytes. The Hs-14 relevant protein does not participate in gamete binding while the Hs-8 detected protein is a secondary binding protein

  • Czech name

    Celistvost akrozomu savčích spermií hodnocená monoklonálními protilátkami proti vnitřním proteinům akrozomu a úloha těchto proteinů ve vazbě spermie a vajíčka

  • Czech description

    Celistvost akrozomu spermie je nezbytná pro normální interakci spermie s vajíčkem. K ověření stavu akrozomu se ještě stále často používají protilátky proti proteinům akrozomu. Studovali jsme akrozomální proteiny pomocí protilátek Hs-8 a Hs-14 připravených v naší laboratoři. Proteiny uvnitř akrozomu rozpoznané Hs-8 a Hs-14 jsou při kapacitaci a dokonce i po indukci akrozomové reakce stabilní. Byla zkoumána úloha proteinů pHs-8 a pHs-14 ve vazbě spermie na vajíčko pomocí vazebného testu kančích spermií aprasečích oocytů in vitro. Protein zjištěny Hs-14 se vazby gamet neúčastní, zatímco protein detekovaný Hs-8 je proteinem sekundární vazby

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EC - Immunology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    American Journal of Reproductive Immunology

  • ISSN

    1046-7408

  • e-ISSN

  • Volume of the periodical

    51

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    2

  • Pages from-to

    480-481

  • UT code for WoS article

  • EID of the result in the Scopus database