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LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00307370" target="_blank" >RIV/68378050:_____/08:00307370 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147

  • Original language description

    Activity of LFA-1 (CD11a/CD18) must be tightly controlled at onset of cellular immunity. Sialoglycoprotein CD43 can affect LFA-1-mediated cell adhesion in anti- or pro-adhesive manner. Using Y2H screen and coimmunoprecipitation we identified and characterized physical and functional association of CD43 with 2 novel partners, LFA-1 and CD147. Monoclonal antibodies to both CD43 and CD147 induced homotypic cell aggregation and adhesion of Jurkat T cells and U937 myeloid cells as well as dynamic co-cappingof LFA-1 together with CD43 and CD147 to cell contact zones. Cotransfection of CD43 interfered with CD147-induced cell adhesion and aggregation, and siRNA-mediated knock down of CD43 in human T cells led to enhanced LFA-1 activation induced via CD147 andT cell antigen receptor. Triggering CD43 and underlying signaling pathways thus seems to enhance LFA-1 adhesiveness. CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147.

  • Czech name

    LFA-1-zprostředkovaná adheze leukocytů je regulována interakcemi CD43 s LFA-1 a CD147

  • Czech description

    Aktivita LFA-1 (CD11a/CD18) musí být při nástupu buněčné imunity důsledně kontrolována. Sialoglykoprotein CD43 může pozitivně či negativně ovlivnit LFA-1-zprostředkovanou buněčnou adhezi. Pomocí Y2H vyhledávání a koimunoprecipitace jsme identifikovali acharakterizovali fyzikální a funkční interakci CD43 se 2 novými partnery, LFA-1 a CD147. Monoklonální protilátky proti CD43 a CD147 indukovaly homotypickou agregaci a adhezi Jurkat T a U937 myeloidních buněk i dynamickou kolokalizaci LFA-1 spolu s CD43 aCD147 na kontaktních zónách buněk. Kotransfekce CD43 interferovala s CD147-indukovanou buněčnou adhezí a agregace a siRNA-zprostředkované potlačení exprese CD43 v lidských T buňkách vedlo ke zvýšené aktivaci LFA-1 indukované přes CD147 a T buněčný receptor. Aktivace CD43 a s ním spojených signálních drah tedy zřejmě zlepšuje adhezivní vlastnosti LFA-1. CD43 ale také negativně reguluje indukci LFA-1 skrze jiné receptory a to dynamickou interakcí s LFA-1 či s CD147.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/1M0506" target="_blank" >1M0506: Center of Molecular and Cellular Immunology</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Molecular Immunology

  • ISSN

    0161-5890

  • e-ISSN

  • Volume of the periodical

    45

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

    000254181800017

  • EID of the result in the Scopus database