Dazap2 modulates transcription driven by the Wnt effector TCF-4

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333973" target="_blank" >RIV/68378050:_____/09:00333973 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Dazap2 modulates transcription driven by the Wnt effector TCF-4

Original language description

A typical feature of the transcriptional response induced by Wnt signaling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signaling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: In association with beta-catenin they activate gene expression, and in the absence of Wnt ligands they act as transcriptional repressors. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. Wedemonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signaling as measured by Tcf/beta-catenin reporters but additionally altered the expression of Wnt signaling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA recognition motif.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000266354600021

EID of the result in the Scopus database

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