A specific type of membrane microdomains is involved in the maintenance and translocation of kinase active Lck to lipid rafts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00377765" target="_blank" >RIV/68378050:_____/12:00377765 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.imlet.2012.01.001" target="_blank" >http://dx.doi.org/10.1016/j.imlet.2012.01.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.imlet.2012.01.001" target="_blank" >10.1016/j.imlet.2012.01.001</a>

Result language
angličtina
Original language name
A specific type of membrane microdomains is involved in the maintenance and translocation of kinase active Lck to lipid rafts
Original language description
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this translocation predicates the subsequent production of IL-2, the mechanism underpinning this process is unknown. Here, we describe the main attributes of this translocating pool of Lck. Using fractionation of Brij58 lysates, derived from primary naive non-activated CD4(+) T cells, we show that a significant portion of Lck is associated with high molecular weight complexes representing a special type of detergent-resistant membranes (DRMs) of relatively high density and sensitivity to laurylmaltoside, thuscalled heavy DRMs. TcR/CD4 coaggregation-mediated activation resulted in the redistribution of more than 50% of heavy DRM-associated Lck to LR in a microtubular network-dependent fashion. Remarkably, in non-activated CD4(+) T-cells, only
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GA310%2F09%2F2084" target="_blank" >GA310/09/2084: Characterization of the molecular machinery regulating the recruitment of signaling molecules to lipid rafts</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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