Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00381590" target="_blank" >RIV/68378050:_____/12:00381590 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gks197" target="_blank" >http://dx.doi.org/10.1093/nar/gks197</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks197" target="_blank" >10.1093/nar/gks197</a>

Result language

angličtina

Original language name

Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site

Original language description

The autonomous transcription of integrated retroviruses strongly depends on genetic and epigenetic effects of the chromatin at the site of integration. These effects are mostly suppressive and proviral activity can be finally silenced by mechanisms, suchas DNA methylation and histone modifications. To address the role of the integration site at the whole-genome-scale, we performed clonal analysis of provirus silencing with an avian leucosis/sarcoma virus-based reporter vector and correlated the transcriptional silencing with the epigenomic landscape of respective integrations. We demonstrate efficient provirus silencing in human HCT116 cell line, which is strongly but not absolutely dependent on the de novo DNA methyltransferase activity, particularlyof Dnmt3b. Proviruses integrated close to the transcription start sites of active genes into the regions enriched in H3K4 trimethylation display long-term stability of expression and are resistant to the transcriptional silencing after o

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

5298-5312

UT code for WoS article

000305829000019

EID of the result in the Scopus database

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