Interleukin 6 signaling regulates promyelocytic leukemia protein gene expression in human normal and cancer cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00390227" target="_blank" >RIV/68378050:_____/12:00390227 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M111.316869" target="_blank" >http://dx.doi.org/10.1074/jbc.M111.316869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M111.316869" target="_blank" >10.1074/jbc.M111.316869</a>
Alternative languages
Result language
angličtina
Original language name
Interleukin 6 signaling regulates promyelocytic leukemia protein gene expression in human normal and cancer cells
Original language description
Tumor suppressor PML is induced under viral and genotoxic stresses by interferons and JAK-STAT signaling. However, the mechanism responsible for its cell type-specific regulation under non-stimulated conditions is poorly understood. To analyze the variation of PML expression, we utilized three human cell types, BJ fibroblasts and HeLa and U2OS cell lines, each with a distinct PML expression pattern. Analysis of JAK-STAT signaling in the three cell lines revealed differences in levels of activated STAT3but not STAT1 correlating with PML mRNA and protein levels. RNAi-mediated knockdown of STAT3 decreased PML expression; both STAT3 level/activity and PML expression relied on IL6 secreted into culture media. We mapped the IL6-responsive sequence to an ISRE(-595/-628) element of the PML promoter. The PI3K/Akt/NF kappa B branch of IL6 signaling showed also cell-type dependence, being highest in BJ, intermediate in HeLa, and lowest in U2OS cells and correlated with IL6 secretion. RNAi-mediat
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA204%2F08%2F1418" target="_blank" >GA204/08/1418: The role of the JAK/STAT signalling pathway in cellular senescence</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Volume of the periodical
287
Issue of the periodical within the volume
32
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
26702-26714
UT code for WoS article
000307386000021
EID of the result in the Scopus database
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