Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00431750" target="_blank" >RIV/68378050:_____/14:00431750 - isvavai.cz</a>
Alternative codes found
RIV/00023752:_____/14:43914573 RIV/00216208:11120/14:43908333
Result on the web
<a href="http://dx.doi.org/10.1016/j.jim.2014.03.007" target="_blank" >http://dx.doi.org/10.1016/j.jim.2014.03.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jim.2014.03.007" target="_blank" >10.1016/j.jim.2014.03.007</a>
Alternative languages
Result language
angličtina
Original language name
Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid
Original language description
This article reports on the development of new sensitive assay for quantification of tau protein. Tau protein in cerebrospinal fluid (CSF) is an important biomarker of Alheimer´s disease and some othe brain diseases. In general, ELISA is usually used fortau protein quantification, but this method is not suitably sensitive. We optimized the use of gold nanoparticles functionalized with tau-specific monoclonal antibody and oligonucleotide template fot immuno-polymerase chain reaction (Nano-iPCR) quantification of tau protein in human CSF samples. We also compared this new method with ELISA, either commercial or newly developed with tyramide signal amplification. Our data offer the superior sensitivity and detection range compared to ELISA in tau proteindetection.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Immunological Methods
ISSN
0022-1759
e-ISSN
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Volume of the periodical
406
Issue of the periodical within the volume
april
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
137-142
UT code for WoS article
000337857600016
EID of the result in the Scopus database
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