Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00436400" target="_blank" >RIV/68378050:_____/14:00436400 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1182/blood-2014-03-564682" target="_blank" >http://dx.doi.org/10.1182/blood-2014-03-564682</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1182/blood-2014-03-564682" target="_blank" >10.1182/blood-2014-03-564682</a>
Alternative languages
Result language
angličtina
Original language name
Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin
Original language description
In non-mammalian vertebrates, the functional units of hemostasis are thrombocytes. Thrombocytes are thought to arise from bi-potent thrombocytic/erythroid progenitors (TEPs). TEPs have been experimentally demonstrated in avian models of hematopoiesis, and mammals possess functional equivalents known as megakaryocyte/erythroid progenitors (MEPs). However, the presence of TEPs in teleosts has only been speculated. To identify and prospectively isolate TEPs, we identified, cloned, and generated recombinantzebrafish thrombopoietin (Tpo). Tpo mRNA expanded itga2b:GFP+ (cd41:GFP+) thrombocytes as well as hematopoietic stem and progenitor cells (HSPCs) in the zebrafish embryo. Utilizing Tpo in clonal methylcellulose assays, we describe for the first time theprospective isolation and characterization of TEPs from transgenic zebrafish. Combinatorial use of zebrafish Tpo, erythropoietin (Epo), and granulocyte colony stimulating factor (Gcsf) allowed the investigation of HSPCs responsible for e
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP305%2F10%2F0953" target="_blank" >GAP305/10/0953: New regulators of megakaryocyte and erythroid lineage commitment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Blood
ISSN
0006-4971
e-ISSN
—
Volume of the periodical
124
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
220-228
UT code for WoS article
000342618600015
EID of the result in the Scopus database
—