Non-essential role for cilia in coordinating precise alignment of lens fibres

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F16%3A00472850" target="_blank" >RIV/68378050:_____/16:00472850 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.mod.2016.01.003" target="_blank" >http://dx.doi.org/10.1016/j.mod.2016.01.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mod.2016.01.003" target="_blank" >10.1016/j.mod.2016.01.003</a>

Result language

angličtina

Original language name

Non-essential role for cilia in coordinating precise alignment of lens fibres

Original language description

The primary cilium, a microtubule-based organelle found in most cells, is a centre for mechano-sensing fluid movement and cellular signalling, notably through the Hedgehog pathway. We recently found that each lens fibre cell has an apically situated primary cilium that is polarised to the side of the cell facing the anterior pole of the lens. The direction of polarity is similar in neighbouring cells so that in the global view, lens fibres exhibit planar cell polarity (PCP) along the equatorial-anterior polar axis. Ciliogenesis has been associated with the establishment of PCP, although the exact relationship between PCP and the role of cilia is still controversial. To test the hypothesis that the primary cilia have a role in coordinating the precise alignment/orientation of the fibre cells, IFT88, a key component of the intraflagellar transport (IFT) complex, was removed specifically from the lens at different developmental stages using several lens-specific Cre-expressing mouse lines (MLR10- and LR-Cre). Irrespective of which Cre-line was adopted, both demonstrated that in IFT88-depleted cells, the ciliary axoneme was absent or substantially shortened, confirming the disruption of primary cilia formation. However no obvious histological defects were detected even when IFT88 was removed from the lens placode as early as E9.5. Specifically, the lens fibres aligned/oriented towards the poles to form the characteristic Y-shaped sutures as normal. Consistent with this, in primary lens epithelial explants prepared from these conditional knockout mouse lenses, the basal bodies still showed polarised localisation at the apical surface of elongating cells upon FGF-induced fibre differentiation. We further investigated the lens phenotype in knockouts of Bardet-Biedl Syndrome (BBS) proteins 4 and 8, the components of the BBSome complex which modulate ciliary function...

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/GA15-23675S" target="_blank" >GA15-23675S: The role of Pax6 in eye development</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mechanisms of Development

ISSN

0925-4773

e-ISSN

—

Volume of the periodical

139

Issue of the periodical within the volume

FEB 2016

Country of publishing house

IE - IRELAND

Number of pages

8

Pages from-to

10-17

UT code for WoS article

000371800400002

EID of the result in the Scopus database

—