Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00487351" target="_blank" >RIV/68378050:_____/17:00487351 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/17:10364900 RIV/00216208:11130/17:10364900 RIV/00064203:_____/17:10364900
Result on the web
<a href="http://dx.doi.org/10.3892/ijmm.2017.3128" target="_blank" >http://dx.doi.org/10.3892/ijmm.2017.3128</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/ijmm.2017.3128" target="_blank" >10.3892/ijmm.2017.3128</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery
Original language description
The nonsyndromic cleft is one of the most frequent congenital defects in humans. Clinical data demonstrated improved and almost scarless neonatal healing of reparative surgery. Based on our previous results on crosstalk between neonatal fibroblasts and adult keratinocytes, the present study focused on characterization of fibroblasts prepared from cleft lip tissue samples of neonates and older children, and compared them with samples isolated from normal adult skin (face and breast) and scars. Although subtle variances in expression profiles of children and neonates were observed, the two groups differed significantly from adult cells. Compared with adult cells, differences were observed in nestin and smooth muscle actin (SMA) expression at the protein and transcript level. Furthermore, fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine, transforming growth factor-beta 1 (TGF-beta 1). Dysregulation of the TGF-beta signalling pathway, including low expression of the TGF-beta receptor II, may contribute to reducing scarring in neonates. Fibroblasts of facial origin also exhibited age independent differences from the cells prepared from the breast, reflecting the origin of the facial cells from neural crest-based ectomesenchyme.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Medicine
ISSN
1107-3756
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
5
Country of publishing house
GR - GREECE
Number of pages
12
Pages from-to
1323-1334
UT code for WoS article
000413398800003
EID of the result in the Scopus database
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