Isl1 Controls Patterning and Mineralization of Enamel in the Continuously Renewing Mouse Incisor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00507049" target="_blank" >RIV/68378050:_____/17:00507049 - isvavai.cz</a>

Result on the web

<a href="https://asbmr.onlinelibrary.wiley.com/doi/full/10.1002/jbmr.3202" target="_blank" >https://asbmr.onlinelibrary.wiley.com/doi/full/10.1002/jbmr.3202</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbmr.3202" target="_blank" >10.1002/jbmr.3202</a>

Result language

angličtina

Original language name

Isl1 Controls Patterning and Mineralization of Enamel in the Continuously Renewing Mouse Incisor

Original language description

Rodents are characterized by continuously renewing incisors whose growth is fueled by epithelial and mesenchymal stem cells housed in the proximal compartments of the tooth. The epithelial stem cells reside in structures known as the labial (toward the lip) and lingual (toward the tongue) cervical loops (laCL and liCL, respectively). An important feature of the rodent incisor is that enamel, the outer, highly mineralized layer, is asymmetrically distributed, because it is normally generated by the laCL but not the liCL. Here, we show that epithelial-specific deletion of the transcription factor Islet1 (Isl1) is sufficient to drive formation of ectopic enamel by the liCL stem cells, and also that it leads to production of altered enamel on the labial surface. Molecular analyses of developing and adult incisors revealed that epithelial deletion of Isl1 affected multiple, major pathways: Bmp (bone morphogenetic protein), Hh (hedgehog), Fgf (fibroblast growth factor), and Notch signaling were upregulated and associated with liCL-generated ectopic enamel, on the labial side, upregulation of Bmp and Fgf signaling, and downregulation of Shh were associated with premature enamel formation. Transcriptome profiling studies identified a suite of differentially regulated genes in developing Isl1 mutant incisors. Our studies demonstrate that ISL1 plays a central role in proper patterning of stem cell-derived enamel in the incisor and indicate that this factor is an important upstream regulator of signaling pathways during tooth development and renewal. (C) 2017 American Society for Bone and Mineral Research.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Bone and Mineral Research

ISSN

0884-0431

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

2219-2231

UT code for WoS article

000417766700008

EID of the result in the Scopus database

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