Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F18%3A00486725" target="_blank" >RIV/68378050:_____/18:00486725 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.nano.2017.09.003" target="_blank" >http://dx.doi.org/10.1016/j.nano.2017.09.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nano.2017.09.003" target="_blank" >10.1016/j.nano.2017.09.003</a>
Alternative languages
Result language
angličtina
Original language name
Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells
Original language description
Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers. (C) 2017 Elsevier Inc. All rights reserved.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nanomedicine: Nanotechnology, Biology and Medicine
ISSN
1549-9634
e-ISSN
—
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
123-130
UT code for WoS article
000423842300012
EID of the result in the Scopus database
—