Novel additions to the CRISPR/Cas tool box beyond SpCas9. Advances in Chemical Biology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00503190" target="_blank" >RIV/68378050:_____/19:00503190 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Novel additions to the CRISPR/Cas tool box beyond SpCas9. Advances in Chemical Biology

Original language description

Clustered regularly interspaced short palindromic repeats (CRISPRs) are found in archaea and many bacteria were they play a role in adaptive immunity against invasion of foreign genetic elements in the form of plasmids or bacteriophages. This bacterial defense machinery acts via specific degradation of the invading nucleic acid by nucleases. In this system, the invading genetic element is recognized by a complex of a short RNA molecule (the guide RNA) and a protein containing nuclease activity. In 2013, the CRISPR/Cas system has been repurposed and converted into a programmable tool for precision genome engineering. The first and most prominent member of the CRISPR/Cas systems employed as a programmable nuclease is Streptococcus pyogenes Cas9 (SpCas9) and it became the best studied and most widely used ribo-nuclease today in a broad range of applications. Despite the great success of Cas9, the search and harnessing of other, alternative orthologue CRISPR systems continued expanding our toolbox for genomic manipulations, transcriptional regulation or sequence specific detection of nucleic acids. Since these newly discovered CRISPR systems complement the functionality and provide interesting alternatives to SpCas9, with promising advantages regarding the size, specificity and additional features, we highlight the new additions to the CRISPR-toolbox in this mini-review.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LO1220" target="_blank" >LO1220: CZ-OPENSCREEN: National infrastructure for chemical biology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Advances in Chemical Biology

ISBN

978-80-88011-03-3

Number of pages of the result

9

Pages from-to

104-113

Number of pages of the book

210

Publisher name

OPTIO CZ

Place of publication

Praha

UT code for WoS chapter

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