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Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00538163" target="_blank" >RIV/68378050:_____/20:00538163 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/20:10422806

  • Result on the web

    <a href="https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000769R" target="_blank" >https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000769R</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1096/fj.202000769R" target="_blank" >10.1096/fj.202000769R</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis

  • Original language description

    Equal segregation of chromosomes during mitosis ensures euploidy of daughter cells. Defects in this process may result in an imbalance in the chromosomal composition and cellular transformation. Proteolytic and non-proteolytic ubiquitylation pathways ensure directionality and fidelity of mitotic progression but specific mitotic functions of deubiquitylating enzymes (DUBs) remain less studied. Here we describe the role of the DUB ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) in the regulation of chromosome bi-orientation and segregation during mitosis. Downregulation or inhibition of UCHL3 leads to chromosome alignment defects during metaphase. Frequent segregation errors during anaphase are also observed upon inactivation of UCHL3. Mechanistically, UCHL3 interacts with and deubiquitylates Aurora B, the catalytic subunit of chromosome passenger complex (CPC), known to be critically involved in the regulation of chromosome alignment and segregation. UCHL3 does not regulate protein levels of Aurora B or the binding of Aurora B to other CPC subunits. Instead, UCHL3 promotes localization of Aurora B to kinetochores, suggesting its role in the error correction mechanism monitoring bi-orientation of chromosomes during metaphase. Thus, UCHL3 contributes to the regulation of faithful genome segregation and maintenance of euploidy in human cells.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    <a href="/en/project/LM2015040" target="_blank" >LM2015040: Czech Centre for Phenogenomics</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FASEB Journal

  • ISSN

    0892-6638

  • e-ISSN

  • Volume of the periodical

    34

  • Issue of the periodical within the volume

    9

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    17

  • Pages from-to

    12751-12767

  • UT code for WoS article

    000558165400001

  • EID of the result in the Scopus database