Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00538163" target="_blank" >RIV/68378050:_____/20:00538163 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10422806
Result on the web
<a href="https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000769R" target="_blank" >https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000769R</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1096/fj.202000769R" target="_blank" >10.1096/fj.202000769R</a>
Alternative languages
Result language
angličtina
Original language name
Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis
Original language description
Equal segregation of chromosomes during mitosis ensures euploidy of daughter cells. Defects in this process may result in an imbalance in the chromosomal composition and cellular transformation. Proteolytic and non-proteolytic ubiquitylation pathways ensure directionality and fidelity of mitotic progression but specific mitotic functions of deubiquitylating enzymes (DUBs) remain less studied. Here we describe the role of the DUB ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) in the regulation of chromosome bi-orientation and segregation during mitosis. Downregulation or inhibition of UCHL3 leads to chromosome alignment defects during metaphase. Frequent segregation errors during anaphase are also observed upon inactivation of UCHL3. Mechanistically, UCHL3 interacts with and deubiquitylates Aurora B, the catalytic subunit of chromosome passenger complex (CPC), known to be critically involved in the regulation of chromosome alignment and segregation. UCHL3 does not regulate protein levels of Aurora B or the binding of Aurora B to other CPC subunits. Instead, UCHL3 promotes localization of Aurora B to kinetochores, suggesting its role in the error correction mechanism monitoring bi-orientation of chromosomes during metaphase. Thus, UCHL3 contributes to the regulation of faithful genome segregation and maintenance of euploidy in human cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/LM2015040" target="_blank" >LM2015040: Czech Centre for Phenogenomics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FASEB Journal
ISSN
0892-6638
e-ISSN
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Volume of the periodical
34
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
12751-12767
UT code for WoS article
000558165400001
EID of the result in the Scopus database
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