Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00539745" target="_blank" >RIV/68378050:_____/20:00539745 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-020-65878-1" target="_blank" >https://www.nature.com/articles/s41598-020-65878-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-020-65878-1" target="_blank" >10.1038/s41598-020-65878-1</a>
Alternative languages
Result language
angličtina
Original language name
Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea
Original language description
Conditional gene targeting in mice by means of Cre-loxP strategy represents a powerful approach to study mammalian gene function. This approach is however dependent on the availability of suitable strains of mice with a tissue or time restricted activity of the Cre recombinase. Here we describe Aldh3-Cre transgenic mice as a useful tool to conditionally delete genes in cornea, a specialized transparent tissue found on the anterior-most part of the eye, which acts as a protective barrier and contributes to the refractive power. Using a set of floxed alleles we demonstrate high Aldh3-Cre activity in corneal epithelial cells, corneal stroma and conjunctival epithelial cells at postnatal stages. Aldh3-Cre will thus be particularly beneficial for functional analysis of genes which are vital for postnatal development of cornea and conjunctiva.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
9083
UT code for WoS article
000543961900021
EID of the result in the Scopus database
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