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Nuclear myosin 1 activates p21 gene transcription in response to DNA damage through a chromatin-based mechanism

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00539824" target="_blank" >RIV/68378050:_____/20:00539824 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.nature.com/articles/s42003-020-0836-1" target="_blank" >https://www.nature.com/articles/s42003-020-0836-1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s42003-020-0836-1" target="_blank" >10.1038/s42003-020-0836-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Nuclear myosin 1 activates p21 gene transcription in response to DNA damage through a chromatin-based mechanism

  • Original language description

    Nuclear myosin 1 (NM1) has been implicated in key nuclear functions. Together with actin, it has been shown to initiate and regulate transcription, it is part of the chromatin remodeling complex B-WICH, and is responsible for rearrangements of chromosomal territories in response to external stimuli. Here we show that deletion of NM1 in mouse embryonic fibroblasts leads to chromatin and transcription dysregulation affecting the expression of DNA damage and cell cycle genes. NM1 KO cells exhibit increased DNA damage and changes in cell cycle progression, proliferation, and apoptosis, compatible with a phenotype resulting from impaired p53 signaling. We show that upon DNA damage, NM1 forms a complex with p53 and activates the expression of checkpoint regulator p21 (Cdkn1A) by PCAF and Set1 recruitment to its promoter for histone H3 acetylation and methylation. We propose a role for NM1 in the transcriptional response to DNA damage response and maintenance of genome stability.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Communications Biology

  • ISSN

    2399-3642

  • e-ISSN

  • Volume of the periodical

    3

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    13

  • Pages from-to

    115

  • UT code for WoS article

    000519367600001

  • EID of the result in the Scopus database