Deletion of TLR2(+) erythro-myeloid progenitors leads to embryonic lethality in mice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00543880" target="_blank" >RIV/68378050:_____/21:00543880 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/eji.202049142" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/eji.202049142</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/eji.202049142" target="_blank" >10.1002/eji.202049142</a>
Alternative languages
Result language
angličtina
Original language name
Deletion of TLR2(+) erythro-myeloid progenitors leads to embryonic lethality in mice
Original language description
Early embryonic hematopoiesis in mammals is defined by three successive waves of hematopoietic progenitors which exhibit a distinct hematopoietic potential and provide continuous support for the development of the embryo and adult organism. Although the functional importance of each of these waves has been analyzed, their spatio-temporal overlap and the lack of wave-specific markers hinders the accurate separation and assessment of their functional roles during early embryogenesis. We have recently shown that TLR2, in combination with c-kit, represents the earliest signature of emerging precursors of the second hematopoietic wave, erythro-myeloid precursors (EMPs). Since the onset of Tlr2 expression distinguishes EMPs from primitive progenitors which coexist in the yolk sac from E7.5, we generated a novel transgenic knock in´mouse model, Tlr2(Dtr) , suitable for inducible targeted depletion of TLR2(+) EMPs. In this model, the red fluorescent protein and diphtheria toxin receptor sequences are linked via a P2A sequence and inserted into the Tlr2 locus before its stop codon. We show that a timely controlled deletion of TLR2(+) EMPs in Tlr2(Dtr) embryos results in a marked decrease in both erythroid as well as myeloid lineages and, consequently, in embryonic lethality peaking before E13.5. These findings validate the importance of EMPs in embryonic development.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Immunology
ISSN
0014-2980
e-ISSN
1521-4141
Volume of the periodical
51
Issue of the periodical within the volume
9
Country of publishing house
DE - GERMANY
Number of pages
14
Pages from-to
2237-2250
UT code for WoS article
000663955300001
EID of the result in the Scopus database
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