Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555595" target="_blank" >RIV/68378050:_____/21:00555595 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/bib/article-abstract/22/6/bbab259/6330938?redirectedFrom=fulltext&login=false" target="_blank" >https://academic.oup.com/bib/article-abstract/22/6/bbab259/6330938?redirectedFrom=fulltext&login=false</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/bib/bbab259" target="_blank" >10.1093/bib/bbab259</a>

Result language
angličtina
Original language name
Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology
Original language description
Significant innovations in next-generation sequencing techniques and bioinformatics tools have impacted our appreciation and understanding of RNA. Practical RNA sequencing (RNA-Seq) applications have evolved in conjunction with sequence technology and bioinformatic tools advances. In most projects, bulk RNA-Seq data is used to measure gene expression patterns, isoform expression, alternative splicing and single-nucleotide polymorphisms. However, RNA-Seq holds far more hidden biological information including details of copy number alteration, microbial contamination, transposable elements, cell type (deconvolution) and the presence of neoantigens. Recent novel and advanced bioinformatic algorithms developed the capacity to retrieve this information from bulk RNA-Seq data, thus broadening its scope. The focus of this review is to comprehend the emerging bulk RNA-Seq-based analyses, emphasizing less familiar and underused applications. In doing so, we highlight the power of bulk RNA-Seq in providing biological insights.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA20-04099S" target="_blank" >GA20-04099S: Molecular characterization of retinal and cerebellar defects caused by mutations in splicing factor Prpf8</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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