A point mutation in human coilin prevents Cajal body formation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00557827" target="_blank" >RIV/68378050:_____/22:00557827 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10456124
Result on the web
<a href="https://journals.biologists.com/jcs/article-abstract/135/8/jcs259587/275105/A-point-mutation-in-human-coilin-prevents-Cajal?redirectedFrom=fulltext" target="_blank" >https://journals.biologists.com/jcs/article-abstract/135/8/jcs259587/275105/A-point-mutation-in-human-coilin-prevents-Cajal?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/jcs.259587" target="_blank" >10.1242/jcs.259587</a>
Alternative languages
Result language
angličtina
Original language name
A point mutation in human coilin prevents Cajal body formation
Original language description
Coilin is a conserved protein essential for integrity of nuclear membrane-less inclusions called Cajal bodies. Here, we report an amino acid substitution (p.K496E) found in a widely-used human EGFP-coilin construct that has a dominant-negative effect on Cajal body formation. We show that this coilin-K496E variant fails to rescue Cajal bodies in cells lacking endogenous coilin, whereas the wild-type construct restores Cajal bodies in mouse and human coilin-knockout cells. In cells containing endogenous coilin, both the wild-type and K496E variant proteins accumulate in Cajal bodies. However, high-level overexpression of coilin-K496E causes Cajal body disintegration. Thus, a mutation in the C-terminal region of human coilin can disrupt Cajal body assembly. Caution should be used when interpreting data from coilin plasmids that are derived from this variant (currently deposited at Addgene).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cell Science
ISSN
0021-9533
e-ISSN
1477-9137
Volume of the periodical
135
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
jcs259587
UT code for WoS article
000798123600011
EID of the result in the Scopus database
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