Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00558437" target="_blank" >RIV/68378050:_____/22:00558437 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0014483522001099?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014483522001099?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exer.2022.109028" target="_blank" >10.1016/j.exer.2022.109028</a>
Alternative languages
Result language
angličtina
Original language name
Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis
Original language description
Iron accumulation has been implicated in degenerative retinal diseases. It can catalyze the production of damaging reactive oxygen species. Previous work has demonstrated iron accumulation in multiple retinal diseases, including age-related macular degeneration and diabetic retinopathy. In mice, systemic knockout of the ferroxidases ceruloplasmin (Cp) and hephaestin (Heph), which oxidize iron, results in retinal iron accumulation and iron-induced degeneration. To determine the role of Heph in the retina, we generated a neural retina-specific Heph knockout on a background of systemic Cp knockout. This resulted in elevated neural retina iron. Conversely, retinal ganglion cells had elevated transferrin receptor and decreased ferritin, suggesting diminished iron levels. The retinal degeneration observed in systemic Cp-/-, Heph- /- mice did not occur. These findings indicate that Heph has a local role in regulating neural retina iron homeostasis, but also suggest that preserved Heph function in either the RPE or systemically mitigates the degeneration phenotype observed in the systemic Cp- /-, Heph-/- mice.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental Eye Research
ISSN
0014-4835
e-ISSN
1096-0007
Volume of the periodical
218
Issue of the periodical within the volume
May
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
109028
UT code for WoS article
000804789500008
EID of the result in the Scopus database
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