TGF beta-induced changes in membrane curvature influence Ras oncoprotein membrane localization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00560339" target="_blank" >RIV/68378050:_____/22:00560339 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-022-17482-8" target="_blank" >https://www.nature.com/articles/s41598-022-17482-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-022-17482-8" target="_blank" >10.1038/s41598-022-17482-8</a>

Result language

angličtina

Original language name

TGF beta-induced changes in membrane curvature influence Ras oncoprotein membrane localization

Original language description

In the course of cancer progression tumor cells undergo morphological changes that lead to increased motility and invasiveness thus promoting formation of metastases. This process called epithelial to mesenchymal transition (EMT) is triggered by transforming growth factor (TGF beta) but for gaining the full invasive potential an interplay between signaling of TGF beta and Ras GTPases is required. Ras proteins possess a lipidated domain that mediates Ras association with the plasma membrane, which is essential for Ras biological functions. Type and number of the lipid anchors are the main difference among three Ras variants-H-ras, N-ras and K-ras. The lipid anchors determine membrane partitioning of lipidated proteins into membrane areas of specific physico-chemical properties and curvature. In this study, we investigated the effect of TGF beta treatment on the subcellular localization of H-ras and K-ras. We show that TGF beta increases positive plasma membrane curvature, which is subsequently sensed by H-ras, leading to its elevated plasma membrane localization and activation. This observation suggests the existence of a novel positive feedback loop whereby the increased level of plasma membrane curvature during TGF beta induced EMT attracts more Ras molecules to the plasma membrane resulting in increased Ras activity which in turn promotes further EMT and thus ultimately enables the acquisition of full invasive potential.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

12

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

13486

UT code for WoS article

000836707300054

EID of the result in the Scopus database

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