QUANTITATIVE ANALYSIS OF SYNCYTIN-1 BINDING TO ITS RECEPTOR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00567915" target="_blank" >RIV/68378050:_____/22:00567915 - isvavai.cz</a>
Result on the web
<a href="http://www.ccsss.cz/index.php/ccsss/issue/view/37/67" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/37/67</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
QUANTITATIVE ANALYSIS OF SYNCYTIN-1 BINDING TO ITS RECEPTOR
Original language description
Syncytin-1 is a fusogenic glycoprotein of retroviral origin that is specifically expressed in the human placenta. Syncytin-1 induces the fusion of cellular membranes of cytotrophoblasts after interaction with its cellular receptor, ASCT2. This process results in the formation of a multi-nuclear syncytiotrophoblast layer, which facilitates the transport of nutrients and metabolites between the mother and the fetus and is required for a successful pregnancy. Mutations in Syncytin-1 or ASCT2 can impair their interaction which may lead to disorders like eclampsia, recurrent pregnancy loss or idiopathic infertility. Up to now, aproper description of the Syncytin-1-ASCT2 binding is missing.We developed a multi-level approach to functionally analyze the efficiency of the Syncytin-1-ASCT2 interaction in a cell system. Ectopically expressed variants of ASCT2 are combined with fluorescent and luminescent proteins and can be instantly detected by microscopy, flow cytometry and common plate readers. We can routinely check for the receptor expression on mRNA and protein levels and validate its localization. We also prepared tools employing Syncytin-1: specific immunoadhesin, infectious virus and reporter cells detecting cellular fusion. With these tools, we can assess ASCT2 ability to bind and prime Syncytin-1.Our system can facilitate precise characterization of the Syncytin-1 binding site on the receptor and lead to detailed molecular understanding of one of the critical steps in human placenta morphogenesis.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů