Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00571281" target="_blank" >RIV/68378050:_____/23:00571281 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/23:00571281 RIV/00216208:11310/23:10468254
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0301462223000406?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0301462223000406?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2023.106989" target="_blank" >10.1016/j.bpc.2023.106989</a>
Alternative languages
Result language
angličtina
Original language name
Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization
Original language description
An advantageous alternative to the use of detergents in biochemical studies on membrane proteins are the recently developed styrene-maleic acid (SMA) amphipathic copolymers. In our recent study [1] we demonstrated that using this approach, most T cell membrane proteins were fully solubilized (presumably in small nanodiscs), while two types of raft proteins, GPI-anchored proteins and Src family kinases, were mostly present in much larger (>250 nm) membrane fragments markedly enriched in typical raft lipids, cholesterol and lipids containing saturated fatty acid residues. In the present study we demonstrate that disintegration of membranes of several other cell types by means of SMA copolymer follows a similar pattern and we provide a detailed proteomic and lipidomic characterization of these SMA-resistant membrane fragments (SRMs).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA19-04047S" target="_blank" >GA19-04047S: Biochemical studies of membrane rafts and immunoreceptors based on cell membrane-disintegrating copolymers</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Chemistry
ISSN
0301-4622
e-ISSN
1873-4200
Volume of the periodical
296
Issue of the periodical within the volume
MAY
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
106989
UT code for WoS article
000961942100001
EID of the result in the Scopus database
2-s2.0-85150788851