ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00573116" target="_blank" >RIV/68378050:_____/23:00573116 - isvavai.cz</a>
Result on the web
<a href="https://www.molbiolcell.org/doi/10.1091/mbc.E22-06-0222" target="_blank" >https://www.molbiolcell.org/doi/10.1091/mbc.E22-06-0222</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1091/mbc.E22-06-0222" target="_blank" >10.1091/mbc.E22-06-0222</a>
Alternative languages
Result language
angličtina
Original language name
ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum
Original language description
Unc-51-like kinase (ULK) family serine-threonine protein kinase homologues have been linked to the function of motile cilia in diverse species. Mutations in Fused/STK36 and ULK4 in mice resulted in hydrocephalus and other phenotypes consistent with ciliary defects. How either protein contributes to the assembly and function of motile cilia is not well understood. Here we studied the phenotypes of ULK4 and Fused gene knockout (KO) mutants in the flagellated protist Leishmania mexicana. Both KO mutants exhibited a variety of structural defects of the flagellum cytoskeleton. Biochemical approaches indicate spatial proximity of these proteins and indicate a direct interaction between the N-terminus of LmxULK4 and LmxFused. Both proteins display a dispersed localization throughout the cell body and flagellum, with enrichment near the flagellar base and tip. The stable expression of LmxULK4 was dependent on the presence of LmxFused. Fused/STK36 was previously shown to localize to mammalian motile cilia, and we demonstrate here that ULK4 also localizes to the motile cilia in mouse ependymal cells. Taken together these data suggest a model where the pseudokinase ULK4 is a positive regulator of the kinase Fused/ STK36 in a pathway required for stable assembly of motile cilia.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology of the Cell
ISSN
1059-1524
e-ISSN
1939-4586
Volume of the periodical
34
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
ar66
UT code for WoS article
001025654800005
EID of the result in the Scopus database
2-s2.0-85159766900