LIPRNAseq: a method to discover lipid interacting RNAs by sequencing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00574412" target="_blank" >RIV/68378050:_____/23:00574412 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11033-023-08548-5#Ack1" target="_blank" >https://link.springer.com/article/10.1007/s11033-023-08548-5#Ack1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11033-023-08548-5" target="_blank" >10.1007/s11033-023-08548-5</a>
Alternative languages
Result language
angličtina
Original language name
LIPRNAseq: a method to discover lipid interacting RNAs by sequencing
Original language description
BackgroundCurrent biological research extensively describes the interactions of molecules such as RNA with other nucleic acids or proteins. However, the relatively recent discovery of nuclear phospholipids playing biologically relevant processes outside membranes, as well as, RNA-lipid interactions shows the need for new methods to explore the identity of these RNAs.Methods and resultsIn this study, we describe the method for LIPID-RNA isolation followed by sequencing and analysis of the RNA that has the ability to interact with the selected lipids. Here we utilized specific phospholipid coated beads for selective RNA binding. We tested RNA from organisms belonging to different realms (human, plant, and yeast), and tested their ability to bind a specific lipid.ConclusionsThe results show several RNAs differentially enriched in the pull-down of phosphatidyl Inositol 4,5 bisphosphate coated beads. This method is helpful to screen lipid-binding RNA, which may have relevant biological functions. The method can be used with different lipids and comparison of pull-downs and can narrow the selection of RNAs that interact with a particular lipid for further studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology Reports
ISSN
0301-4851
e-ISSN
1573-4978
Volume of the periodical
50
Issue of the periodical within the volume
8
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
6619-6626
UT code for WoS article
001014466700002
EID of the result in the Scopus database
2-s2.0-85162619331