Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: <i>In vitro</i>-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00579877" target="_blank" >RIV/68378050:_____/24:00579877 - isvavai.cz</a>

Result on the web

<a href="https://www.spandidos-publications.com/10.3892/or.2023.8662" target="_blank" >https://www.spandidos-publications.com/10.3892/or.2023.8662</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2023.8662" target="_blank" >10.3892/or.2023.8662</a>

Result language

angličtina

Original language name

Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: <i>In vitro</i>-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling

Original language description

Breast cancer is the most frequently diagnosed cancer in women worldwide. Although dramatically increased survival rates of early diagnosed cases have been observed, late diagnosed patients and metastatic cancer may still be considered fatal. The present study's main focus was on cancer-associated fibroblasts (CAFs) which is an active component of the tumor microenvironment (TME) regulating the breast cancer ecosystem. Transcriptomic profiling and analysis of CAFs isolated from breast cancer skin metastasis, cutaneous basal cell carcinoma, and squamous cell carcinoma unravelled major gene candidates such as IL6, VEGFA and MFGE8 that induced co-expression of keratins-8/-14 in the EM-G3 cell line derived from infiltrating ductal breast carcinoma. Western blot analysis of selected keratins (keratin-8,14,18,19) and epithelial-mesenchymal transition-associated markers (SLUG, SNAIL, ZEB1, E-/N-cadherin, vimentin) revealed specific responses pointing to certain heterogeneity of the studied CAF populations. Experimental in vitro treatment using neutralizing antibodies against IL-6, VEGF-A and MFGE8 attenuated the modulatory effect of CAFs on EM-G3 cells. The present study provided novel data in characterizing and understanding the interactions between CAFs and EM-G3 cells in vitro. CAFs of different origins support the pro-inflammatory microenvironment and influence the biology of breast cancer cells. This observation potentially holds significant interest for the development of novel, clinically relevant approaches targeting the TME in breast cancer. Furthermore, its implications extend beyond breast cancer and have the potential to impact a wide range of other cancer types.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncology Reports

ISSN

1021-335X

e-ISSN

1791-2431

Volume of the periodical

51

Issue of the periodical within the volume

1

Country of publishing house

GR - GREECE

Number of pages

12

Pages from-to

3

UT code for WoS article

001111760900001

EID of the result in the Scopus database

2-s2.0-85177454254