An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00597892" target="_blank" >RIV/68378050:_____/24:00597892 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/24:00137205
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2211124724009689?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211124724009689?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.celrep.2024.114618" target="_blank" >10.1016/j.celrep.2024.114618</a>
Alternative languages
Result language
angličtina
Original language name
An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation
Original language description
Adar null mutant mouse embryos die with aberrant double-stranded RNA (dsRNA)-driven interferon induction, and Adar Mavs double mutants, in which interferon induction is prevented, die soon after birth. Protein kinase R (Pkr) is aberrantly activated in Adar Mavs mouse pup intestines before death, intestinal crypt cells die, and intestinal villi are lost. Adar Mavs Eifak2 (Pkr) triple mutant mice rescue all defects and have longterm survival. Adenosine deaminase acting on RNA 1 (ADAR1) and PKR co-immunoprecipitate from cells, suggesting PKR inhibition by direct interaction. AlphaFold studies on an inhibitory PKR dsRNA binding domain (dsRBD)-kinase domain interaction before dsRNA binding and on an inhibitory ADAR1 dsRBD3PKR kinase domain interaction on dsRNA provide a testable model of the inhibition. Wild-type or editing- inactive human ADAR1 expressed in A549 cells inhibits activation of endogenous PKR. ADAR1 dsRNA binding is required for, but is not sufficient for, PKR inhibition. Mutating the ADAR1 dsRBD3-PKR contact prevents co-immunoprecipitation, ADAR1 inhibition of PKR activity, and co-localization of ADAR1 and PKR in cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cell Reports
ISSN
2211-1247
e-ISSN
2211-1247
Volume of the periodical
43
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
25
Pages from-to
114618
UT code for WoS article
001297074700001
EID of the result in the Scopus database
2-s2.0-85201161071