Molecular mechanisms of proteoglycan-mediated semaphorin signaling in axon guidance.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00604436" target="_blank" >RIV/68378050:_____/24:00604436 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10489568 RIV/00216224:90127/24:00139058
Result on the web
<a href="https://www.pnas.org/doi/epdf/10.1073/pnas.2402755121" target="_blank" >https://www.pnas.org/doi/epdf/10.1073/pnas.2402755121</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2402755121" target="_blank" >10.1073/pnas.2402755121</a>
Alternative languages
Result language
angličtina
Original language name
Molecular mechanisms of proteoglycan-mediated semaphorin signaling in axon guidance.
Original language description
The precise assembly of a functional nervous system relies on axon guidance cues. Beyond engaging their cognate receptors and initiating signaling cascades that modulate cytoskeletal dynamics, guidance cues also bind components of the extracellular matrix, notably proteoglycans, yet the role and mechanisms of these interactions remain poorly understood. We found that Drosophila secreted semaphorins bind specifically to glycosaminoglycan (GAG) chains of proteoglycans, showing a preference based on the degree of sulfation. Structural analysis of Sema2b unveiled multiple GAG-binding sites positioned outside canonical plexin-binding site, with the highest affinity binding site located at the C-terminal tail, characterized by a lysine-rich helical arrangement that appears to be conserved across secreted semaphorins. In vivo studies revealed a crucial role of the Sema2b C-terminal tail in specifying the trajectory of olfactory receptor neurons. We propose that secreted semaphorins tether to the cell surface through interactions with GAG chains of proteoglycans, facilitating their presentation to cognate receptors on passing axons.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
1091-6490
Volume of the periodical
121
Issue of the periodical within the volume
31
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"e2402755121"
UT code for WoS article
001397018400015
EID of the result in the Scopus database
2-s2.0-85200152595