Genetic background affects neutrophil activity and determines the severity of autoinflammatory osteomyelitis in mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00617297" target="_blank" >RIV/68378050:_____/24:00617297 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10489796

Result on the web

<a href="https://academic.oup.com/jleukbio/article/117/1/qiae168/7730917" target="_blank" >https://academic.oup.com/jleukbio/article/117/1/qiae168/7730917</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jleuko/qiae168" target="_blank" >10.1093/jleuko/qiae168</a>

Result language

angličtina

Original language name

Genetic background affects neutrophil activity and determines the severity of autoinflammatory osteomyelitis in mice

Original language description

The knowledge about the contribution of the innate immune system to health and disease is expanding. However, to obtain reliable results, it is critical to select appropriate mouse models for in vivo studies. Data on genetic and phenotypic changes associated with different mouse strains can assist in this task. Such data can also facilitate our understanding of how specific polymorphisms and genetic alterations affect gene function, phenotypes, and disease outcomes. Extensive information is available on genetic changes in all major mouse strains. However, comparatively little is known about their impact on immune response and, in particular, on innate immunity. Here, we analyzed a mouse model of chronic multifocal osteomyelitis, an autoinflammatory disease driven exclusively by the innate immune system, which is caused by an inactivating mutation in the Pstpip2 gene. We investigated how the genetic background of BALB/c, C57BL/6J, and C57BL/6NCrl strains alters the molecular mechanisms controlling disease progression. While all mice developed the disease, symptoms were significantly milder in BALB/c and partially also in C57BL/6J when compared to C57BL/6NCrl. Disease severity correlated with the number of infiltrating neutrophils and monocytes and with the production of chemokines attracting these cells to the site of inflammation. It also correlated with increased expression of genes associated with autoinflammation, rheumatoid arthritis, neutrophil activation, and degranulation, resulting in altered neutrophil activation in vivo. Together, our data demonstrate striking effects of genetic background on multiple parameters of neutrophil function and activity influencing the onset and course of chronic multifocal osteomyelitis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Leukocyte Biology

ISSN

0741-5400

e-ISSN

1938-3673

Volume of the periodical

117

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

qiae168

UT code for WoS article

001320122000001

EID of the result in the Scopus database

2-s2.0-85214318789