Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00617533" target="_blank" >RIV/68378050:_____/24:00617533 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-024-72418-8" target="_blank" >https://www.nature.com/articles/s41598-024-72418-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-024-72418-8" target="_blank" >10.1038/s41598-024-72418-8</a>
Alternative languages
Result language
angličtina
Original language name
Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines
Original language description
The International Mouse Phenotyping Consortium (IMPC) systematically produces and phenotypes mouse lines with presumptive null mutations to provide insight into gene function. The IMPC now uses the programmable RNA-guided nuclease Cas9 for its increased capacity and flexibility to efficiently generate null alleles in the C57BL/6N strain. In addition to being a valuable novel and accessible research resource, the production of 3313 knockout mouse lines using comparable protocols provides a rich dataset to analyze experimental and biological variables affecting in vivo gene engineering with Cas9. Mouse line production has two critical steps generation of founders with the desired allele and germline transmission (GLT) of that allele from founders to offspring. A systematic evaluation of the variables impacting success rates identified gene essentiality as the primary factor influencing successful production of null alleles. Collectively, our findings provide best practice recommendations for using Cas9 to generate alleles in mouse essential genes, many of which are orthologs of genes linked to human disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
22626
UT code for WoS article
001326080400042
EID of the result in the Scopus database
2-s2.0-85205446158