Transcription elongation factor ELOF1 is required for efficient somatic hypermutation and class switch recombination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00619325" target="_blank" >RIV/68378050:_____/25:00619325 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.molcel.2025.02.007" target="_blank" >https://doi.org/10.1016/j.molcel.2025.02.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molcel.2025.02.007" target="_blank" >10.1016/j.molcel.2025.02.007</a>
Alternative languages
Result language
angličtina
Original language name
Transcription elongation factor ELOF1 is required for efficient somatic hypermutation and class switch recombination
Original language description
Somatic hypermutation (SHM) and class switch recombination (CSR) diversify immunoglobulin (Ig) genes and are initiated by the activation-induced deaminase (AID), a single-stranded DNA cytidine deaminase thought to engage its substrate during RNA polymerase II (RNAPII) transcription. Through a genetic screen, we identified numerous potential factors involved in SHM, including elongation factor 1 homolog (ELOF1), a component of the RNAPII elongation complex that functions in transcription-coupled nucleotide excision repair (TC-NER) and transcription elongation. Loss of ELOF1 compromises SHM, CSR, and AID action in mammalian B cells and alters RNAPII transcription by reducing RNAPII pausing downstream of transcription start sites and levels of serine 5 but not serine 2 phosphorylated RNAPII throughout transcribed genes. ELOF1 must bind to RNAPII to be a proximity partner for AID and to function in SHM and CSR, and TC-NER is not required for SHM. We propose that ELOF1 helps create the appropriate stalled RNAPII substrate on which AID acts.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Cell
ISSN
1097-2765
e-ISSN
1097-4164
Volume of the periodical
85
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
"1296"-"1310.e7"
UT code for WoS article
001468627800001
EID of the result in the Scopus database
2-s2.0-105001146360