Mouse X-linked microRNA cluster regulates the meiotic checkpoint and Prdm9- driven hybrid sterility in a copy number-dependent manner
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00642626" target="_blank" >RIV/68378050:_____/25:00642626 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/25:00642626
Result on the web
<a href="https://doi.org/10.1073/pnas.2510229122" target="_blank" >https://doi.org/10.1073/pnas.2510229122</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2510229122" target="_blank" >10.1073/pnas.2510229122</a>
Alternative languages
Result language
angličtina
Original language name
Mouse X-linked microRNA cluster regulates the meiotic checkpoint and Prdm9- driven hybrid sterility in a copy number-dependent manner
Original language description
One of the reproductive barriers between diverging populations during formation of a new species is the sterility of their hybrids. The Prdm9-driven hybrid male sterility of Mus musculus musculus × Mus musculus domesticus hybrids depends on the interaction between PRDM9, a histone methyltransferase that determines the positions of meiotic recombination hotspots, and an as yet unknown X-linked genetic factor within the Hybrid sterility X2 (Hstx2) locus. Here, we report that the Mir465 microRNA (miRNA) gene cluster is the predicted Hstx2 hybrid sterility factor. We show that removal of the Mir465 genes restores the fertility of sterile hybrids and improves meiotic synapsis of homologous chromosomes. Mir465 knockout also restores spermatogenesis in sterile chromosomal translocation carriers, demonstrating that Mir465 acts as a meiotic checkpoint that can be activated independently of Prdm9 intersubspecific incompatibility. Furthermore, the Mir465 knockout increases the global recombination rate in hybrids and in parental Mus m. domesticus mice. This demonstrates that Mir465 is responsible for the phenotypes of the two overlapping genetic loci, the Hstx2 engaged in fertility of hybrids and the Meiotic recombination 1 (Meir1) controlling the recombination rate. The finding of enlarged Mir465 clusters in all European Mus m. musculus samples tested and the identification of differentially expressed targets suggest that the reproductive barrier between the two subspecies is sensitive to copy number variation of Mir465 genes. Together, the underdominant interaction between Prdm9 and Mir465 provides a rare example of Dobzhansky–Muller incompatibility in hybrids of closely related species, making it accessible for further analysis at the molecular level.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
1091-6490
Volume of the periodical
122
Issue of the periodical within the volume
40
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
2510229122
UT code for WoS article
001600401100001
EID of the result in the Scopus database
2-s2.0-105017590339