Optimizing atomic force microscopy for characterization of diamond-protein interfaces

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F11%3A00375015" target="_blank" >RIV/68378271:_____/11:00375015 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/1556-276X-6-337" target="_blank" >http://dx.doi.org/10.1186/1556-276X-6-337</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1556-276X-6-337" target="_blank" >10.1186/1556-276X-6-337</a>

Result language

angličtina

Original language name

Optimizing atomic force microscopy for characterization of diamond-protein interfaces

Original language description

Atomic force microscopy (AFM) in contact mode and tapping mode is employed for high resolution studies of soft organic molecules (fetal bovine serum proteins) on hard inorganic diamond substrates in solution and air. Various effects in morphology and phase measurements related to the cantilever spring constant, amplitude of tip oscillations, surface approach, tip shape and condition are demonstrated and discussed based on the proposed schematic models. We show that both diamond and proteins can be mechanically modified by Si AFM cantilever. We propose how to choose suitable cantilever type, optimize scanning parameters, recognize and minimize various artifacts, and obtain reliable AFM data both in solution and in air to reveal microscopic characteristics of protein-diamond interfaces. We also suggest that monocrystalline diamond is well defined substrate that can be applicable for fundamental studies of molecules on surfaces in general.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BM - Solid-state physics and magnetism

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanoscale Research Letters

ISSN

1931-7573

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

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Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

"337/1"-"337/10"

UT code for WoS article

000292289900005

EID of the result in the Scopus database

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