Proton-induced direct and indirect damage of plasmid DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F15%3A00448614" target="_blank" >RIV/68378271:_____/15:00448614 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/15:00448614 RIV/68407700:21340/15:00235108
Result on the web
<a href="http://dx.doi.org/10.1007/s00411-015-0605-6" target="_blank" >http://dx.doi.org/10.1007/s00411-015-0605-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00411-015-0605-6" target="_blank" >10.1007/s00411-015-0605-6</a>
Alternative languages
Result language
angličtina
Original language name
Proton-induced direct and indirect damage of plasmid DNA
Original language description
Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Radiation and Environmental Biophysics
ISSN
0301-634X
e-ISSN
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Volume of the periodical
54
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
343-352
UT code for WoS article
000358328800010
EID of the result in the Scopus database
2-s2.0-84938996562