DLC films as material suitable for adhesion of biomolecules from Luria Bertani medium

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F17%3A00486995" target="_blank" >RIV/68378271:_____/17:00486995 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DLC films as material suitable for adhesion of biomolecules from Luria Bertani medium
Original language description
Diamond-like carbon (DLC) films are promising for medical applications, especially due to their antibacterial properties. In this work, the adhesion of biomolecules from complex cultivation medium Luria Bertani (LB) on surface terminated DLC films is investigated using X-ray photoelectron spectroscopy (XPS).The DLC films were deposited by DC PACVD method. Their surface termination by H, O or F was carried out by RF plasma in appropriate gas atmosphere (hydrogen, oxygen, carbon tetrafluoride). All functionalized samples were sterilized in autoclave before immersion into LB medium. AFM measurements confirmed the adhesion of ~2 nm thin peptide layer on LB-treated samples. XPS measurements revealed the differences in NH2/NH3 ratios for LB-treated glass and LB-DLC films. In case of oxygen, the dependence of O-H/C=O bond ratio on the surface termination was observed. These indicate that the adhesion of biomolecules from LB medium can be affected by DLC surface termination.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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