Electrospray sample injection for single-particle imaging with x-ray lasers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00521634" target="_blank" >RIV/68378271:_____/19:00521634 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0306226" target="_blank" >http://hdl.handle.net/11104/0306226</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/sciadv.aav8801" target="_blank" >10.1126/sciadv.aav8801</a>

Result language
angličtina
Original language name
Electrospray sample injection for single-particle imaging with x-ray lasers
Original language description
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10305 - Fluids and plasma physics (including surface physics)

Project
<a href="/en/project/LQ1606" target="_blank" >LQ1606: ELI Beamlines: International Center of Excelence</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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